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Macrogen rna microarray analysis
Rna Microarray Analysis, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+microarray-analysis/analysis+microarray/pm40829387-106-0-7
Average 86 stars, based on 1 article reviews
rna microarray analysis - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Isolation:

Article Title: TNF promotes osteoclastogenesis by secreting miR-31-5p into small extracellular vesicles via the autotaxin-LPA-LPAR1 axis in arthritic fibroblast-like synoviocytes.
Article Snippet: Fibroblast-like synoviocytes (FLSs) play a crucial role in the pathogenesis of arthritis.. However, the impact of small extracellular vesicles (sEVs) secreted by FLSs on osteoclastogenesis remains incompletely understood.. In this study, we aimed to investigate the role of tumor necrosis factor (TNF)and lysophosphatidic acid (LPA)activated FLSs in sEV-mediated release of osteoclastogenic miRNAs and elucidate their functional contribution to osteoclastogenesis.

Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells
Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for miRNA microarray analysis by Macrogen Inc. .. The miRNA microarray system with Affymetrix GeneChip ® 4.0 array (Affymetrix; Thermo Fisher Scientific, Inc.) containing 2,578 human mature miRNA oligonucleotide probes was used according to the manufacturer's recommended protocol (Affymetrix; Thermo Fisher Scientific, Inc.).

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea). .. The Affymetrix Genechip miRNA array process was performed according to the manufacturer’s protocol.

Microarray:

Article Title: TNF promotes osteoclastogenesis by secreting miR-31-5p into small extracellular vesicles via the autotaxin-LPA-LPAR1 axis in arthritic fibroblast-like synoviocytes.
Article Snippet: Fibroblast-like synoviocytes (FLSs) play a crucial role in the pathogenesis of arthritis.. However, the impact of small extracellular vesicles (sEVs) secreted by FLSs on osteoclastogenesis remains incompletely understood.. In this study, we aimed to investigate the role of tumor necrosis factor (TNF)and lysophosphatidic acid (LPA)activated FLSs in sEV-mediated release of osteoclastogenic miRNAs and elucidate their functional contribution to osteoclastogenesis.

Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells
Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for miRNA microarray analysis by Macrogen Inc. .. The miRNA microarray system with Affymetrix GeneChip ® 4.0 array (Affymetrix; Thermo Fisher Scientific, Inc.) containing 2,578 human mature miRNA oligonucleotide probes was used according to the manufacturer's recommended protocol (Affymetrix; Thermo Fisher Scientific, Inc.).

Article Title: Predicting treatable traits for long-acting bronchodilators in patients with stable COPD
Article Snippet: .. RNA microarray analysis was performed for 31,426 gene transcripts by a specialized company, Macrogen (Seoul, South Korea). ..

Article Title: New insights into the olfactory development disrupted by 4-tert-Octylphenol in offspring mice.
Article Snippet: • Maternal 4-tert-octylphenol (4t-OP) exposure reduced subventricular zone

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea). .. The Affymetrix Genechip miRNA array process was performed according to the manufacturer’s protocol.

Transfection:

Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells
Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for miRNA microarray analysis by Macrogen Inc. .. The miRNA microarray system with Affymetrix GeneChip ® 4.0 array (Affymetrix; Thermo Fisher Scientific, Inc.) containing 2,578 human mature miRNA oligonucleotide probes was used according to the manufacturer's recommended protocol (Affymetrix; Thermo Fisher Scientific, Inc.).

Control:

Article Title: RAS-stimulated release of exosomal miR-494-3p promotes the osteolytic bone metastasis of breast cancer cells
Article Snippet: The cells were seeded in a cell culture dish (2.5×10 5 cells/ml), cultured for 24 h, and then transfected with nucleic acids (plasmids, 1 μ g per transfection; siRNAs or miRNAs, 10 or 100 μ M) using Lipofectamine 3000 (for plasmids, cat. no. L3000-015) or RNAiMAX (for siRNAs and miRNAs, cat. no. 13778-150) according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.) After 48 h, the transfected cells were used for further experiments. .. Exosomes isolated from the MCF-7 cells transfected with control or KRASV12 were used for miRNA microarray analysis by Macrogen Inc. .. The miRNA microarray system with Affymetrix GeneChip ® 4.0 array (Affymetrix; Thermo Fisher Scientific, Inc.) containing 2,578 human mature miRNA oligonucleotide probes was used according to the manufacturer's recommended protocol (Affymetrix; Thermo Fisher Scientific, Inc.).

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea). .. The Affymetrix Genechip miRNA array process was performed according to the manufacturer’s protocol.

Plasmid Preparation:

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Article Snippet: Samples were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA). .. MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea). .. The Affymetrix Genechip miRNA array process was performed according to the manufacturer’s protocol.



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Image Search Results


A) Percent mature and star miRNAs with m 6 A methylation marks. B-D) m 6 A methylation patterns in each treatment group. E-G) Heatmap of m6A methylation profile versus miRNA abundance.

Journal: bioRxiv

Article Title: Cannabinoids shift the basal ganglia miRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected Rhesus macaques

doi: 10.1101/2024.10.11.614514

Figure Lengend Snippet: A) Percent mature and star miRNAs with m 6 A methylation marks. B-D) m 6 A methylation patterns in each treatment group. E-G) Heatmap of m6A methylation profile versus miRNA abundance.

Article Snippet: We have completed the Arraystar Human N6-methyladenosine (m6A) small RNA modification microarray analysis of the samples you submitted.

Techniques: Methylation

A) Neuroinflammation network molecules associated with the with miRNAs with m6A hypomethylation (left) or hypermethylation (right).

Journal: bioRxiv

Article Title: Cannabinoids shift the basal ganglia miRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected Rhesus macaques

doi: 10.1101/2024.10.11.614514

Figure Lengend Snippet: A) Neuroinflammation network molecules associated with the with miRNAs with m6A hypomethylation (left) or hypermethylation (right).

Article Snippet: We have completed the Arraystar Human N6-methyladenosine (m6A) small RNA modification microarray analysis of the samples you submitted.

Techniques: